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I've found that if i do that, it creates a gap between the top of the motor shaft, and the bottom of the lead screw. This then causes the aluminium spiral type connector to compress under the weight of the x axis carriage assembly, causing inaccuracy on that axis. To prevent this, i have allowed the lead screw to fall under gravity until it stops on top of the motor shaft, then nipped up the grub screws. The down side to this, is that the lead screw is then 8 mm below the top acrylic support.