MakerBot Print is our newest print-prepration software, which supports native CAD files and STL assemblies,
allows you to interact with all your printers via the Cloud, and many other exciting new features.
I think you can as long as you keep the same ratio for both pulleys and idlers. Of course you need to set up your firmware accordingly too. Regarding precision, you're right, using 16th pulleys give you a slightly better precision (10 micron instead of 12.5 micron).